Journal: Oncology Letters

Article Title: Analysis of PKC-ζ protein levels in normal and malignant breast tissue subtypes

doi: 10.3892/ol.2018.9792

Figure Lengend Snippet: PKC-ζ knockdown retards invasion of MDA-MB-231 breast cancer cells. MDA-MB-231 breast cancer cells were placed in the upper chamber of Transwell plate coated with 0.5X BME and serum containing media was placed in the lower chamber as a chemoattractant. Following transfection with siPRKCZ and scrambled RNA, cells that invaded through the BME and migrated into the lower chamber were stained with crystal violet and (A) observed under a microscope at magnification, ×10; scale bar, 1 mm. Untreated cells and cells treated with the transfection reagent (Si-Tran) were also used to establish the effect on invasion. (B) The cells were counted for each treatment image ( N =3), averaged and analyzed with the standard deviation, a Student's t-test (*P<0.05) and a one-way ANOVA was also used to evaluate the data with the post-hoc Tukey's HSD test (P<0.01), Scheffé multiple comparison (P<0.01), Bonferroni (P<0.01) and Holm (P<0.01). All statistical tests were used to determine the statistical significance of the invasion data. (C) The cells were further investigated by western blot to analyze the Rac1/RhoA pathway. This data was quantified and averaged for the graph with standard error of the mean represented. PKC-ζ, protein kinase C-ζ; BME, basement membrane extract; Rac1, Ras-related C3 botulinum toxin substrate 1; RhoA, Ras homolog gene family member A.

Article Snippet: Human breast cancer cells MDA-MB-231 were grown in 100 mm plates and transfected with 20 nM of scrambled RNA and siPRKCZ (5′-GCAUGAUGACGAGGAUAUUGACUGG-3′, SR303747A; Origene, Rockville, MD, USA) for 48 h. Cells were lysed as previously described and the lysates were run on western blots.

Techniques: Transfection, Staining, Microscopy, Standard Deviation, Western Blot

Journal: Oncology Letters

Article Title: Analysis of PKC-ζ protein levels in normal and malignant breast tissue subtypes

doi: 10.3892/ol.2018.9792

Figure Lengend Snippet: Organization of F-actin upon the knockdown of PKC-ζ. MDA-MB-231 breast cancer cells were treated with SiTran, scrambled RNA and siPRKC-ζ for 24 h. The cells that received no treatment were used as the control. These cells were then stained with 1X Phalloidin-iFluor 594 and counterstained with DAPI. Scale bar, 100 µm. Original magnification, ×20. DAPI, 4′,6-diamidino-2-phenylindole; F-actin, filamentous actin; PKC-ζ, protein kinase C-ζ.

Article Snippet: Human breast cancer cells MDA-MB-231 were grown in 100 mm plates and transfected with 20 nM of scrambled RNA and siPRKCZ (5′-GCAUGAUGACGAGGAUAUUGACUGG-3′, SR303747A; Origene, Rockville, MD, USA) for 48 h. Cells were lysed as previously described and the lysates were run on western blots.

Techniques: Staining

Journal: Scientific Reports

Article Title: Programmed activation of cancer cell apoptosis: A tumor-targeted phototherapeutic topoisomerase I inhibitor

doi: 10.1038/srep29018

Figure Lengend Snippet: ( a–f ) Intracellular fluorescence intensity recorded for A549 cells 1 h after incubation with of PT-1 in the absence ( a–c ) of and ( d–f ) after irradiation with 405 nm light for 1 h over the area delineated by the yellow dashed line. ( g ) Cytotoxicity of PT-1 observed in two cancer cell lines (A549 and HeLa) and two human normal fibroblast cell lines (WI-38 and BJ) after photo-activation. ( h ) Cell viability (relative percentage) observed for the A549, HeLa, WI-38, and BJ cell lines after treatment with PT-1 (incubation for 1 h) followed by irradiation at 405 nm for 1 h. Viability was determined via a 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay performed 24 h after photo-irradiation. *P < 0.05. ( i–l ) Phase contrast images of A549 cancer cells undergoing apoptosis. In these studies, the cells were incubated with 10 nM PT-1 for 30 min followed by irradiation with 405 nm laser light for 1 h over the area indicated by the yellow dashed lines ( j , l ).

Article Snippet: Twelve biotin receptor-positive cell lines; human lung carcinoma cells (A549), human cervical cancer cells (HeLa), human breast cancer cells (MCF7, MDA-M231), human liver cancer cells (HepG2, Huh7, Hep3B), human prostate cancer cells (Du145, PC3), human gastric cancer cells (NCI-N87, AGS), human pancreatic cancer cell (Panc-1), and a biotin receptor-negative cell: human normal embryonal kidney epithelial cell (293T) were purchased from the Korean Cell Line Bank (Seoul, Republic of Korea).

Techniques: Fluorescence, Incubation, Irradiation, Activation Assay, MTT Assay

Journal: Sensors (Basel, Switzerland)

Article Title: A Novel Microwave Resonant Sensor for Measuring Cancer Cell Line Aggressiveness

doi: 10.3390/s22124383

Figure Lengend Snippet: Mean ± SD of resonant frequency, min|S 11 (f)|, full width at half maximum, and fitting error. n = 8.

Article Snippet: Pediatric human osteosarcoma cell line SaOS-2 (HTL01001), human breast adenocarcinoma cell lines MCF7 (HTL95021) and MDA-MB-231 (HTL99004) were purchased from Banca Biologica and Cell Factory (IRCCS Azienda Ospedaliera Universitaria San Martino-IST, Genova, Italy), while the pediatric osteosarcoma cell line 143B (CRL-8303) was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: A Novel Microwave Resonant Sensor for Measuring Cancer Cell Line Aggressiveness

doi: 10.3390/s22124383

Figure Lengend Snippet: Resonant frequency values computed for pure DMEM, and the four tested cell lines: low-aggressive SaOS-2 and high-aggressive 143B osteosarcoma cell lines, and low-aggressive MCF7 and high-aggressive MDA-MB-231 breast cancer cell lines. n = 8. *, p < 0.05 and ****, p < 0.0001.

Article Snippet: Pediatric human osteosarcoma cell line SaOS-2 (HTL01001), human breast adenocarcinoma cell lines MCF7 (HTL95021) and MDA-MB-231 (HTL99004) were purchased from Banca Biologica and Cell Factory (IRCCS Azienda Ospedaliera Universitaria San Martino-IST, Genova, Italy), while the pediatric osteosarcoma cell line 143B (CRL-8303) was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: A Novel Microwave Resonant Sensor for Measuring Cancer Cell Line Aggressiveness

doi: 10.3390/s22124383

Figure Lengend Snippet: Min|S 11 (f)| ( a ) and FWHM ( b ) computed for pure DMEM, and the four tested cell lines: low-aggressive SaOS-2 and high-aggressive 143B osteosarcoma cell lines, and low-aggressive MCF7 and high-aggressive MDA-MB-231 breast cancer cell lines. n = 8.

Article Snippet: Pediatric human osteosarcoma cell line SaOS-2 (HTL01001), human breast adenocarcinoma cell lines MCF7 (HTL95021) and MDA-MB-231 (HTL99004) were purchased from Banca Biologica and Cell Factory (IRCCS Azienda Ospedaliera Universitaria San Martino-IST, Genova, Italy), while the pediatric osteosarcoma cell line 143B (CRL-8303) was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: